Figure 5.

Single-cell RNA sequencing analysis reveals distinct immune cell compositions between KPPC and PPSSC pancreatic tumors

(A) Single-cell RNA sequencing (scRNA-seq) analysis of live CD45⁺ immune cell mixture from KPPC and PPSSC tumors. The major immune cell clusters were shown in the uniform manifold approximation and projection (UMAP) plot.

(B and C) UMAP plot comparing the immune cell compositions between KPPC and PPSSC tumors (B). UMAP distributions of immune cell clusters were also compared between KPPC and PPSSC tumors (C).

(D) Comparison of the relative abundance (%) of various immune cell clusters between KPPC and PPSSC tumors.

(E) Dot plot showing the expression profiles of representative marker genes for indicated cell clusters.

(F) Myeloid cells from KPPC and PPSSC groups were stratified into two distinct subclusters, myeloid-1 and myeloid-2, in the UMAP plot. The abundance (%) of myeloid-1 and myeloid-2 subclusters was also shown in the pie chart.

(G) Expression profiles of signature genes for the two myeloid subpopulations, shown as violin plots.

(H) The cell-cell communication network across indicated immune cell subclusters was calculated and visualized using a circular plot.

(I) The communication levels of cytokine-mediated outgoing (Out) or incoming (In) signaling pathways associated with myeloid-1 and myeloid-2 subclusters in KPPC tumors and PPSSC tumors. See also Figure S8.