REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit CK19	Abcam	Cat# ab52625, RRID: AB_2281020
Rabbit Ki67	Abcam	Cat# ab15580, RRID: AB_443209
Goat Col1	SouthernBiotech	Cat# 1310-01, RRID: AB_2753206
Mouse αSMA	DAKO	Cat# M0851, RRID: AB_2223500
Rabbit CD4	Abcam	Cat# ab183685, RRID: AB_2686917
Rabbit CD8	Cell Signaling	Cat# 85336S, RRID: AB_2800052
Rabbit Arginase 1	Abcam	Cat# ab91279, RRID: AB_10674215
Rabbit CD45	Cell Signaling	Cat# 70257, RRID: AB_2799780
Rat CK8	Sigma	Cat# MABT329, RRID: AB_2891089
Rabbit Cadherin 1	Cell Signaling	Cat# 3195, RRID: AB_2291471
Rabbit Vimentin	Cell Signaling	Cat# 5741, RRID: AB_10695459
Rabbit ZEB1	Novus Biological	Cat# NBP1-05987, RRID: AB_1556166
Rabbit phospho-ERK1/2	Cell Signaling	Cat# 9101S, RRID: AB_331646
Rabbit ERK	Cell Signaling	Cat# 9102, RRID: AB_330744
Rabbit Cleaved Caspase-3	Cell Signaling	Cat# 9664S, RRID: AB_2070042
Rabbit p63	Abcam	Cat# ab124762, RRID: AB_10971840
Rabbit β-actin	Cell Signaling	Cat# 4970, RRID: AB_2223172
Rabbit CK5	Cell Signaling	Cat#71536T, RRID: AB_3101753
Rabbit HSP90	Cell Signaling	Cat# 4877T, RRID: AB_2233307
Goat CD206	R&D Systems	Cat# AF2535, RRID: AB_2063012
Rabbit CK5	BioLegend	Cat# 905501, RRID: AB_2565050
Mouse CK8	Abcam	Cat# ab9023, RRID: AB_306948
Rat Ly6G	Abcam	Cat# ab25377, RRID: AB_470492
Rabbit CD4	Cell Signaling	Cat# 25229, RRID: AB_2798898
Rat FoxP3	eBioscience	Cat# 4-4771-80, RRID: AB_529583
Rat Granzyme B	eBioscience	Cat# 50-8898-82, RRID: AB_11219679
Rat LAG3	Bio X Cell	Cat# BP0174, RRID: AB_10949602
Rat PD-1	Bio X Cell	Cat# BE0273, RRID: AB_2687796
Bacterial and virus strains
pCMV5B-Smad4	Addgene	Cat# 11743
Chemicals, peptides, and recombinant proteins
Protease inhibitor	Roche	Cat# 4693116001
Crystal violet	Sigma	Cat# C0775
Lipofectamine 2000	Invitrogen	Cat# 11668027
RNAiMAX	Invitrogen	Cat# 13778-150
MRTX1133	Selleck	Cat# E1051
GSK1120212	Selleck	Cat# S2673
Verteporfin	Selleck	Cat# S1786
Gemcitabine	LC Laboratories	Cat# G4199
Niclosamide	Selleck	Cat# S3030
Olaparib	Selleck	Cat# S1060
JQ1	Selleck	Cat# S7110
MK-2206 2HCl	Selleck	Cat# S1078
7RH	MedChemExpress	Cat# HY-U00444
VS-4718	Selleck	Cat# S7653
Rapamycin	Selleck	Cat# S1039
RSL3	MedChemExpress	Cat# HY100218A
Erastin	MedChemExpress	Cat# HY15763
Collagenase IV	Gibco	Cat# 17104019
Dispase II	Gibco	Cat# 17105041
Type I collagen solution from rat tail	Sigma-Aldrich	Cat# C3867
Critical commercial assays
Direct-zol RNA Kit	Zymo Research	Cat# 11-331
Reverse Transcription Kit	Applied Biosystems	Cat# 4368814
SYBR Green Master Mix	Applied Biosystems	Cat# 4367659
Cell Counting Kit-8	Abcam	Cat# ab228554
Chromium Single Cell 3′ Reagent Kits (v2)	10x Genomics	Cat# PN-120237
ABC-Kit	Vector	Cat# PK-6100
Stable DAB	Invitrogen	Cat# 750118
Alcian blue Stain Kit	Abcam	Cat# 150662
Pierce BCA protein assay kit	Thermo Fisher	Cat# 23208
Picrosirius Red	Abcam	Cat# 150681
TruSeq Stranded mRNA Sample Prep Kit	Illumina	Cat# 20020594
Deposited data
KPPC and PPSSC cancer cell lines RNA-sequencing	This paper	GEO: GSE268896
KPPC and PPSSC pancreatic tumors Single-cell RNA-sequencing	This paper	GEO: GSE268899
TCGA pancreatic adenocarcinoma cohort survival and gene expression data (GDAC Firehose PAAD)	Broad Institute	http://gdac.broadinstitute.org/runs/stddata__2016_01_28/data/PAAD/20160128/
Experimental models: Cell lines
Primary mouse KPPC cancer cell line	This paper	N/A
Primary mouse PPSSC cancer cell line	This paper	N/A
Experimental models: Organisms/strains
Mouse: LSL-KrasG12D/+;Trp53loxP/+;Pdx1-Cre	Chen et al.32	N/A
Mouse: LSL-KrasG12D/+;Trp5R172H/+;Pdx1-Cre	Chen et al.32	N/A
Mouse: Smad4loxP/loxP	Jackson Laboratory	017462
Mouse: Tgfbr2loxP/loxP	Jackson Laboratory	012603
Oligonucleotides
See Table S1	Sigma	N/A
Recombinant DNA
pCMV5B-Smad4	Addgene	Cat# 11743
Software and algorithms
Prism v10.0.0	GraphPad Software Inc.	http://www.graphpad.com/
cBioportal v2.2.0	MSK Center for Mol Onc	https://www.cbioportal.org/
Seurat R package (3.5.3)	Satija et al.34	https://satijalab.org/seurat/
DESeq2	Anders and Huber35	https://bioconductor.org/packages/release/bioc/html/DESeq2.html
STAR aligner	Dobin et al.36	https://github.com/alexdobin/STAR
Gene Set Enrichment Analysis (GSEA)	Broad Institute	http://software.broadinstitute.org/gsea/index.jsp