Figure 3.

mRNA-encoded anchored IDO1 is protective in EAE

(A) Mice were induced with EAE with MOG peptide and pertussis toxin (PT) and then injected i.v. with 0.5 mg/kg of LNP A-formulated mRNA on days −1 and 6.

(B and C) Mean clinical score (B) and percentage of disease-free mice (C) were tracked over time.

(D and E) Mean disease intensity (D) and mean peak score (E) were calculated between days 10 and 21.

(B) Data are mean and SEM of 12 mice/group and representative of 4 similar experiments. Significance was determined compared to dead SRC.IDO by two-way ANOVA with a secondary Dunnett’s multiple comparisons test. ∗∗p < 0.01 and #p < 0.0001. (C) Significance was determined by log-rank (Mantel-Cox) test to dead SRC.IDO. ∗∗∗∗p < 0.0001. (D and E) Data are individual mice and median of 12 mice/group and representative of 4 similar experiments. Significance was determined by one-way ANOVA with secondary Tukey’s multiple comparisons test. ∗∗∗∗p < 0.0001.