Figure 5.

Src-anchored IDO1 delivered by mRNA protects host B cells in aGVHD

(A) C57BL/6xDBA/2 F1 recipients received C57BL/6 donor cells and were injected i.v. with 0.5 mg/kg of LNP A-formulated mRNA on days 0 and 6.

(B–E) Donor CD8 T cell engraftment (B and D) and host B cell (C and E) percentages were determined by flow cytometry on (B and C) day 7 in peripheral blood and (D and E) day 14 in the spleen.

(F and G) On day 14, splenic CD4 and CD8 T cells within the donor and host compartments were analyzed for expression of (F) active caspase-3 (apoptosis) or (G) Ki67 (proliferation).

(H) The percentages of CD4 T cells expressing FoxP3 within the spleen (top) and blood (bottom) were determined to quantify Treg populations. Data are individuals and median. Data are of n = 3–8 mice/group and representative of 3 similar experiments. Significance was determined by one-way ANOVA with a secondary Tukey’s multiple comparisons test. ∗∗p < 0.005, ∗∗∗p < 0.0005, and ∗∗∗∗p < 0.0001.