REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-IDO1 (D727U) rabbit mAb	Cell Signaling Technology	Cat#68572 RRID:AB_2799750
anti-HSP90 (C45G5)	Cell Signaling Technology	Cat#4877 RRID:AB_2233307
IRDYE 800 CW goat anti-rabbit	LI-COR Biosciences	Cat#926-32211 RRID:AB_621843
TruStain FcX (anti-mouse CD16/32) antibody	Biolegend	Cat#101320 RRID:AB_1574975
BV805 Hamster anti-mouse CD3 (clone 145-2C11)	BD Biosciences	Cat#749276 RRID:AB_2873651
Brilliant Violet 785 Rat anti-mouse CD4 (clone GK1.5)	Biolegend	Cat#100453 RRID:AB_2565843
BD Horizon BUV737 Rat anti-mouse CD8a (clone 53–6.7)	BD Biosciences	Cat#612759 RRID:AB_2870090
Alexa Fluor 700 Rat anti-mouse CD19 (clone 6D5)	Biolegend	Cat#115528 RRID:AB_493735
PE MHC class I (H-2Kd) anti-mouse (clone SF1-1.1.1)	Invitrogen	Cat#12-5957-82 RRID:AB_2043875
Brilliant Violet 421 (H-2Kb) anti-mouse (clone AF6-88.5)	Biolegend	Cat#116525 RRID:AB_2876430
Alexa Fluor 647 anti-mouse/rat/human FoxP3 (clone 150D)	Biolegend	Cat#320014 RRID:AB_439750
Brilliant Violet 605 Rat anti-mouse Ki-67 (clone 16A8)	Biolegend	Cat#652413 RRID:AB_2562664
FitC Rabbit anti-Active Caspase-3	BD Biosciences	Cat#559341 RRID:AB_397234
Biological samples
Heat Inactivated fetal bovine serum certified one shot	Gibco	Cat#A38400-01
Chemicals, peptides, and recombinant proteins
Dulbeco’s Modified Media (DMEM)	Gibco	Cat#11965-092
MOG35-55	AnaSpec	Cat#AS-60130-1
CFA with 2.5 mg/mL Mycobacterium tuberculosis H37Ra	BD Biosciences	Cat#211372
Pertussis toxin	List Labs	Cat#180
Chicken collagen type II	Sigma	Cat#C9301
IFA	BD Biosciences	Cat#210486
RIPA lysis buffer	Thermo Fisher Scientific	Cat#89901
NuPage 4–12% Bis-Tris Midi gels	Invitrogen	Cat#WG1403BX10
iBlot 2 nitrocellulose regular stacks membranes	Invitrogen	Cat#IB23001
Odyssey LI-COR Blocking Buffer	Li-Corbio	Cat#927-60001
10x lysis buffer	Cell Signaling Technology	Cat#9803
7mm stainless steel bead	Qiagen	Cat#69990
70-μm cell strainer	Celltreat	Cat#229484
K2 EDTA-coated tubes	Griener Bio One	Cat#450532
ACK lysis buffer	Gibco	Cat#A1049201
PBS pH 7.4 (1x)	Gibco	Cat#10010-023
Invitrogen eBioscience Foxp3/Transcription Factor Staining Buffer Set	Invitrogen	Cat#05-5523-00
Leica Bond Epitope retrieval solution 1	Leica Biosystems	Cat#AR0086
Rabbit monoclonal anti-V5 antibody	Cell Signaling Technology	Cat#13202
Bond Polymer Refine Detection system	Lecia Biosystems	Cat#DS9914
Invitrogen eBioscience Fixable Viability Dye eFluor 780	Invitrogen	Cat#65086514
20mM Tris Buffer with 8% sucrose, pH 7.4	Teknova	Cat#3T6100
Critical commercial assays
Pierce BCA kit	Thermo Fisher Scientific	Cat#23227
Tryptophan Competitive Enzyme Immunoassay	IBL-America	Cat#IB89127R
Kynurenine Competitive Enzyme Immunoassay	IBL-America	Cat#IB89190R
Human IDO ELISA Kit	Abcam	Cat#ab245710
Experimental models: Cell lines
Raw 264.7	ATCC	Cat# TIB-71 RRID:CVCL_0493
Experimental models: Organisms/strains
C57Bl/6 (female)	Charles River Laboratories	Cat#027 RRID:IMSR_CRL:027
B6D2F1 (female)	Charles River Laboratories	Cat#099 RRID:IMSR_CRL:099
Sprague-Dawley rats (male)	Charles River Laboratories	Cat#001 RRID:RGD_734476
Software and algorithms
FlowJo	BD Life Science	https://www.flowjo.com/
Graphpad Prism 10.0	Graphpad	https://www.graphpad.com/
ImageJ	ImageJ	https://imagej.net/ij/
HALO image analysis software	Indica Labs	https://indicalab.com/