Figure 2.

Probing the substrate tolerance of Clostridium perfringens sialidases using the acetylated sialosides microarray. The acetylated sialosides array was treated with NanH_GH33, NanI_GH33, and NanJ_GH33 (20 μg/ml in pH 7.4 PBS buffer) at 37 °C for 1 h. Bovine coronavirus hemagglutinin-esterase (BCoV HE) was then used to probe the result of the sialidase treatment. Loss of binding signal after sialidase treatment suggests cleavage of the sialic acid. Microarray data for the untreated control and the NanH_GH33, NanI_GH33, and NanJ_GH33 treated slides are shown for (A) acetylated 2,3-sialosides, (B) acetylated 2,6-sialosides, and (C) acetylated 2,8-sialosides. Data are shown as quadruplicate values, with the error bar representing standard deviation of the mean. D, summary of substrate tolerance of the sialidase on the acetylated sialosides.