Figure 7.

CE-LIF-based substrate reduction assays reveal activity against α2,3/6-linked sialosides for NanH_GH33, NanI_GH33, and NanJ_GH33. Mixtures of fluorogenically labeled 3’/6′-SL contain lactose (Lac) which was removed from the assay mixture by pretreatment with β-galactosidase to yield labeled glucose (Glc). Neuraminidases were added and immediately analyzed by CE-LIF, the first time point reflecting the 2 min capillary preconditioning prior to sample injection. CE-LIF, capillary electrophoresis with laser induced flourescence.