Table 1.
Kinetic parameters for uninhibited and inhibited hydrolysis of X-Neu5Ac
| Kinetic constant | NanH | NanI | NanJ |
|---|---|---|---|
| Vmax (μM/s) | 0.13 ± 0.01 | 0.09 ± 0.01 | 0.13 ± 0.01 |
| Km (μM) | 280.0 ± 62.7 | 123.0 ± 25.4 | 87.0 ± 10.7 |
| kcat (1/s) | 13.3 ± 1.2 | 9.2 ± 0.6 | 15.3 ± 0.6 |
| kcat/Km | 0.05 ± 0.01 | 0.08 ± 0.02 | 0.18 ± 0.02 |
| Ki (μM) | |||
| DANA | 33.9 ± 4.4 | 3.1 ± 0.5 | 3.2 ± 0.3 |
| Siastatin B | 125.0 ± 16.2 | 10.7 ± 2.0 | 13.3 ± 1.6 |
| Peramivir | ND | ND | 104.0 ± 28.4 |
| Zanamivir | ND | ND | ND |
| Oseltamivir | ND | ND | ND |
Standard error is shown. ND indicates inhibition too weak to be detected or quantified.