Extended Data Fig. 6. i3D cylinder incorporation within the closing neural tube.
a, b. Representative embryo (of 4 independent embryos cultured overnight) showing encircling of an i3D cylinder by the surface ectoderm and neural folds, leaving an open defect, shown as maximum projections (A) and 3D confocal reconstructions (B). Note the persistent apical enrichment of phalloidin-labelled neuroepithelial F-actin. Scale bar = 100 μm. c. Live-imaging of a CellMask-labelled embryos (representative of 6 independent embryos with stiff cylinders) showing progression of rostral-to-caudal zippering (*) until it encircles a stiff cylinder incorporated within its neural folds. Note triangle and square shapes incorporated in the cylinder illustrating control over printed geometries and lack of rotation. Scale bar = 100 μm.