Extended Data Fig. 9. iMeSH cylinders can be directly attached to tissues.
a. Apical surface of an iPSC-derived neuroepithelial cell layer showing cortical F-actin expected to be under tension (magenta springs). Scale bar = 10 μm. b. Laser ablation of a vertical line in the apical surface of a live-imaged iPSC-derived neuroepithelium. Arrows indicate displacement visualised by PIV. Scale bar = 50 μm. c, d. iMeSH cylinders were printed attached to the apical surface of iPSC-derived neuroepithelial cells (C) or chick embryos (representative of 7 embryos with laser ablations with printed cylinders) (D). A straight laser ablation (dashed red line) is created in the neuroepithelium and the iMeSH re-imaged immediately after. Merged and PIV visualisation illustrate cylinder expansion, absorbing elastic energy released from the ablation site. Images are representative of 6 independent wells. Green fluorescence along the ablation line is present even without i3D polymer and likely reflects a change in laser-ablated CellMaskTM used to visualise cell borders. Scale bars = 50 μm.