Extended Data Fig. 4. Influence of GATC containing-sequences on downstream-MPRA expression.

Examination of the effect of GATC motif’s impact on MPRA expression levels using 6-mers. For all 26 unique 6-mer sequences containing a GATC, including their reverse complements, the average log₂ expression difference between sequences having the 6-mer and those lacking the 6-mer is plotted. 6-mers are ordered based on their median effect across the eight experimental setups. Shape indicates the construct backbone (p35S or pTRP1), and color represents the host species. The six 6-mers (CAgatc, AgatcT, AgatcC, gatcTA, gatcCA, and AgatcG) with the highest median effect were used to establish the consensus YVGATCBR sequence defined as the GATC motif, which was then used in downstream analyses.