Fig. 4.

In vitro anti-tumor evaluation. (A) Intracellular ROS detection for 4T1 cells treated with PBS, IR890 or P-IR890 by using DCFH-DA probe. The nucleus of treated cancer cells were stained with DAPI. (Scale bar: 50 µm) (B) Cell viability of 4T1 cells treated with varying concentrations of IR890 or P-IR890 without light irradiation. (C) Cell viability of 4T1 cells treated with varying concentrations of IR890 or P-IR890 and irradiated with deep NIR light.(D) Live/dead staining images for 4T1 cells with different treatments. (Scale bar: 100 µm).