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. 2024 Oct 30;7:1295. doi: 10.1038/s42003-024-06281-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Reference signature (middle—thalamus, striatum, and cerebellum top to bottom, respectively) based ground truth annotation (right) in mouse brain ST data (scale bar = 500 µm). b MILWRM, SpaGCN, GraphST, PRECAST, and BASS detected tissue domains (k = 13) overlaid on three mouse brain ST samples. c Proportion of tissue domains in slides each bar corresponding to the legend on the left. d Scatter plot for ARI and consensus ARI. e Correlation matrix for overall correlation between MILWRM and reference scores for each TD and anatomical region across all spots.