Fig. 2.

WA could reduce the accumulation of LDs in the hippocampus of mice with postoperative cognitive impairment. (A) Experimental schematic. Ten-month-old mice were administered WA at doses of 1, 2, and 4 mg/kg, three times per week, for a duration of two months. Subsequently, a tibial fracture model was established to induce postoperative cognitive impairment in mice. Behavioral and biological assessments were conducted thereafter. (B) Fluorescence staining of hippocampal microglia cells and detection of LDs accumulation using BODIPY probe (scale bar = 10 μm, n = 3). Average fluorescence intensity of BODIPY-labeled LDs and droplet count depicted in (C) and (D), respectively. Hippocampal area Oil Red staining (scale bar = 100–50 μm, n = 3), with statistical representation shown in (E, F). *P < 0.05, **P < 0.01, ***P < 0.001 compared with the corresponding group, as determined by the one-way ANOVA .