Fig. 2.

POM and POLFM coating conditions showed improved adherence, neurite development and smaller aggregates of iPSC-MNs. (A) Representative morphology of the cells grown on dishes pre-coated with POL, POLF, POLFM or POM (from left to right) and images were taken on day 19, 24, and 28 (from top to bottom). Scale bar 100 μm. (B) The quantification of aggregate size among four coatings on day 28 of differentiation. N = 3 biological replicates. The data were presented as mean ± SEM. Variations among groups were examined for statistical significance using unpaired student’s t test. *p < 0.05, **p < 0.01, and ***p < 0.001.