Fig. 3.

PEI coating condition promoted an even distribution of iPSC-MNs on the MEA plates in an Supplementary culture. (A-B) Representative morphology of iPSC-MN cultures on day 18, day 30 and day 48 from 48-well MEA plates coated with POM (A_a’-c’) or PEI (B_a’-c’). The top panels were taken under 4 × magnification, and the bottom panels were taken from the respective well on the top but under 10 × magnification. Scale bar, 100 µM. (C) Plots of MEA recording data for the number of active electrodes from the longitudinal recording of day 18 to 48 of iPSC-MNs on the POM or PEI-coating conditions. The active electrodes was defined as ≥ 5 spikes/min. The total recorded wells were n = 24 in the POM-coating condition and n = 45 in the PEI coating condition from three independent cell lines. Data were presented as mean ± SEM. Variations among groups were examined for statistical significance using multiple unpaired student’s t test. *p < 0.05, **p < 0.01 and ***p < 0.001. See also Table S2.