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. 2024 Oct 30;14:26106. doi: 10.1038/s41598-024-77710-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — The PEI and POM coating enabled detection of hyperexcitability of iPSC-MNs from sALS patients. Plots of MEA recording data for the number of active electrodes (A, B), mean firing rate (Hz) (C, D), network burst frequency (E, F) and weighted mean firing rate (G, H) from the longitudinal recording of day 18 to 48 of iPSC-MNs on the PEI or POM-coating conditions. The total recorded wells were n = 45 for the PEI coating condition and n = 24 for the POM-coating condition from three independent cell lines. The data were presented as the mean ± SEM. Variations among groups were examined using multiple unpaired student’s t test. *p < 0.05, **p < 0.01 and ***p < 0.001. See also Fig. S4 and Tables S3–S4.