Fig. 5.

Effects of ebeiedinone and peimisine on CSE-induced cell apoptosis in BEAS-2B cells. BEAS-2B cells were co-incubated with ebeiedinone, peimisine (1, 10, 40, and 80 μM) and CSE for 24 h. (a) and (b) Cells were stained with Annexin V-FITC and PI and flow cytometrically analyzed for cell apoptosis. (c) and (g) The levels of apoptotic proteins were detected by a western blot assay. (d) and (h) The quantified expression of Bax/Bcl-2. (e) and (i) The quantified expression of PARP/GAPDH. (f) and (j) The quantified expression of Cleaved PARP/GAPDH. The data are represented as the mean ± standard deviation of three independent experiments. ^###P < 0.001 and ^####P < 0.0001 compared with the control group. **P < 0.01, ***P < 0.001, and ****P < 0.0001 compared with the CSE group. Abbreviation used: ns, not significant.