Fig. 4.

Lipid flux analyses of host-derived lipids in the TgACS3-iKD mutant under high host nutrient content. Using 13°C stable isotope-prelabelled host cells, scavenged 13C-lipid profile and abundance channeled into the parasite membranes were quantified by GCMS approach for A: total FA, B: TAG, C: FFA, D: DAG, and E: PL fractions in high nutrient content, that is 10% FBS. Error bars indicate standard deviation (SD), Experiments were conducted in triplicates, unpaired t test P values where 0.01–0.05 = ∗, 0.01–0.001 = ∗∗, <0.001 = ∗∗∗ and <0.0001 = ∗∗∗∗.