Table 1.
PCR primers used in this study
| TgACS Gene ID | Primers (Gene specific sequence underlined) | Molecular system used |
|---|---|---|
| TgACS3 (TGGT1_310080) | 5′ flank Fw: GGGCGCGCCGGATCCTTAATTAATCGCTGCGATGTCGATCGTTTTC | TATI-Tet off |
| 5′ flank Rw: CGCCATGCATGGCCGGCCCATATGGCGACTACGAAAGACAAACGCC | ||
| 3′ flank Fw: TGTTCCAGATTATGCCTTACCCGGGATGGCACTCCAGTACGCCTACC | ||
| 3′ flank Rw: TGGAGCTCCACCGCGGTGGCGGCCGCCTGCTTCGAGGAGAATGGCTTTC | ||
| SD01: CGATGACCTGTGTCGACCTGT | ||
| SD02: TCTTCTTTGAGGGAAGAGGAAACG | ||
| SD03: GGTACCGAGCTCGACTTTCAC | ||
| TgACS1 (TGGT1_297220) | Fw: TACTTCCAATCCAATTTAATGCAGCCTGGGGATGCCGATCAACT Rw: TCCTCCACTTCCAATTTTAGCCAGCTTTGCCTGCAGCGC |
pLIC HA tagging |
| TgACS2 (TGGT1_310150) | Fw: TACTTCCAATCCAATTTAATGCGCGCTCGGCGTTGAGTT Rw: TCCTCCACTTCCAATTTTAGCCGACCACCACGACCGCA |
pLIC HA tagging |
| TgACS4 (TGGT1_243800) | Fw: TACTTCCAATCCAATTTAATGCCTTGCTTGGTGGCCATCATCG Rw: TCCTCCACTTCCAATTTTAGCAATCGCCTTCGCTCTCTCCG |
pLIC HA tagging |
| TgACS5 (TGGT1_247760) | Fw: TACTTCCAATCCAATTTAATGCCCTCGGATCATCGACCGAGC Rw: TCCTCCACTTCCAATTTTAGCATTGGCAGGATGGTGCCGT |
pLIC HA tagging |
| TgACS6 (TGGT1_232580) | Fw: TACTTCCAATCCAATTTAATGCGAAGATGAGATGACCGGGC Rw: TCCTCCACTTCCAATTTTAGCCTTATCTTCGACGTCCTTTACAG |
pLIC HA tagging |
| TgACS7 (TGGT1_276155) | Fw: TACTTCCAATCCAATTTAATGCGAAGCAGGCGAACCTGGA Rw: TCCTCCACTTCCAATTTTAGCGTCGTCGTTCTTCAAAAGTTCG |
pLIC HA tagging |