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. 2024 Oct 30;26:149. doi: 10.1186/s13058-024-01900-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — A Overlapping box and jitter plots depicting GSVA estimation scores for cAMP/PKA/CREB signaling related pathways in ILC and ILC like (red; ILC—HCC2185, MDAMB134, MDAMB330, SUM44PE; ILC like—MPE600, CAMA1, HCC2218, MDAMB453, OCUBM, SKBR5, ZR7530) and NST (blue; NST—BT474, BT483, EFM19, HCC1008, HCC1419, HCC1500, LY2, MCF7, MDAMB157, MDAMB175, T47D, UACC812, ZR75-1, ZR75B) cell lines based on gene expression data from Marcotte et al. 2016; GOBP response to cAMP = GOBP cellular response to cAMP, GOBP regulation of cAMP = GOBP regulation of cAMP mediated signaling and KEGG cAMP signaling = KEGG cAMP mediated signaling. B Overlapping box and jitter plots depicting ratio of quantified levels of western blot for CREB, p-CREB and PKACA in ILC/ILC-like and NST cell lines before and after treatment with forskolin (each normalized for corresponding levels of β-actin); ILC and ILC-like cell lines (red; MPE600, WCRC25, ZR75-30, MM134, CAMA-1, BCK4, SUM44PE) and NST cell lines (blue; MCF7, T47D, BT474, EFM19, ZR75-1); Y-axis represents the ratio of levels of CREB, p-CREB, and PKACA with forskolin treatment to their baseline levels (vehicle treatment). C Western blot images for levels of CREB, p-CREB, and PKACA in ILC and ILC-like (red; MPE600, WCRC25, ZR75-30, MM134, CAMA-1, BCK4, SUM44PE) and NST (blue; MCF7, T47D, BT474, EFM19, ZR75-1) cell lines with Vehicle (Veh) and Forskolin (Fors) treatment. D Overlapping box and jitter plots depicting ratio of quantified levels of western blot for CREB, p-CREB and PKACA in patient-derived organoids (PDOs) before and after treatment with forskolin (each normalized for corresponding levels of β-actin): ILC PDOs (PDO146 PDO86 PDO53 PDO46 PDO30) and NST PDOs (PDO126 PDO56 PDO134 PDO123 PDO111 PDO102 PDO129); Y-axis represents the ratio of levels of CREB, p-CREB, and PKACA with forskolin treatment to their baseline levels (vehicle treatment). GSVA—Gene Set Variation Analysis; ILC—Invasive lobular breast cancer; NST—Invasive carcinoma—no special type; PKA—Protein kinase A; PKACA—Protein kinase A catalytic α subunit; CREB—cAMP Response Element Binding protein; p-CREB—phospho CREB, phosphorylated at Ser133; KEGG—Kyoto Encyclopedia of Genes and Genomes; GOBP—Gene Ontology Biological Process; PDO—Patient Derived Organoids