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. 2024 Oct 31;13:80. doi: 10.1186/s40249-024-01255-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — The establishment of colorimetric pp-IPA assay for malaria detection. A fivefold dilution series made from lysate of a cultured 3D7 standard strain was assayed using colorimetric pp-IPA. The results were observed at 0, 20, and 40 min, respectively. The left image shows an overview of pp-IPA amplification, with the red box indicating the amplification result observation area and the black rectangle indicating the no template control (NTC). The right image is an enlarged view of the result observation area. The concentrations ranged from 0.000128 to 2 parasites/μl and were marked above each sample. The associated RT-qPCR and RT-LAMP results for each sample were listed as well for comparison. Each sample was run in triplicates and one representative pipette was shown. pp-IPA Pasteur pipette-assisted isothermal probe amplification, ND not detected