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. 2024 Oct 31;13:80. doi: 10.1186/s40249-024-01255-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Specificity of pp-IPA. The specificity of pp-IPA was examined by amplifying DENV1, DENV2, DENV3, DENV4, ZIKV, CHIKV, and JEV using malaria CPs, LPs, and primers. Blank Pasteur pipette (no oligo conjugated) and NTC were set as negative controls. The left image shows an overview of pp-IPA, with the red box indicating the amplification result observation area and the black rectangle indicating the positive control for P. falciparum. The right image is an enlarged view of the amplification result observation area. Each sample was run in triplicates and one representative pipette was shown. The associated Cq values of DENV1, DENV2, DENV3, DENV4, ZIKV, CHIKV, JEV and P. falciparum from viral- and Plasmodium-specific RT-qPCR were 18.6 (± 0.4), 23.8 (± 0.2), 15.9 (± 0.5), 21.6 (± 0.4), 23.0 (± 0.7), 22.9 (± 0.1), 19.1 (± 0.6), 19.3 (± 0.2), respectively. pp-IPA Pasteur pipette-assisted isothermal probe amplification, DENV1 dengue virus serotype 1, DENV2 dengue virus serotype 2, DENV3 dengue virus serotype 3, DENV4 dengue virus serotype 4, ZIKV Zika virus, CHIKV chikungunya virus, JEV Japanese encephalitis virus, NTC no template control