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. 2024 Oct 31;13:80. doi: 10.1186/s40249-024-01255-8

Fig. 5.

Fig. 5

Clinical samples validation by pp-IPA. Eight clinical samples were amplified using pp-IPA, the red rectangle indicates the area containing the IPA mix for result readout, and color change of reaction mix was observed at 0, 20 and 40 min three time points. The Cq values of each sample, as determined by RT-qPCR were recorded below the sample ID numbers. Each sample was run in triplicates and one representative pipette was shown. pp-IPA Pasteur pipette-assisted isothermal probe amplification, ND not detected