Table 2. Primers used for RT-qPCR assays.
| Name | Sequence (5′–3′) | Hybridization Temp (°C) | Length nt | References |
|---|---|---|---|---|
| Standard genes | ||||
| rpoD (10F) | CAGCAATCACGCGTTAAAGA | 60 | 144 | Mauffrey, Martineau & Villemur (2015) |
| rpoD (153R) | ACCCAGGTCGCTGAACATAC | |||
| rpob (3861F) | TGAGATGGAGGTTTGGGCAC | 60 | 146 | Mauffrey, Martineau & Villemur (2015) |
| rpob (4006R) | GCATACCTGCATCCATCCGA | |||
| dnaG (774F) | CATCCTGATCGTGGAAGGTT | 60 | 121 | Mauffrey, Martineau & Villemur (2015) |
| dnaG (894R) | GCTGCGAATCAACTGACGTA | |||
| Regulatory genes | ||||
| narX1 (1403F) | TGCTGAAGCCCTACAAGTGG | 60 | 133 | This study |
| narX1 (1535R) | TGCGTTAGCGATAGCACCTT | |||
| narL1 (174F) | ATGCCGGGAATAGGAGGAGT | 60 | 136 | This study |
| narL1 (309R) | AATAACCGCGGGCACCATTA | |||
| fnr2 (121F) | ACCGGCTATGTCTACCGTTG | 60 | 149 | This study |
| fnr2 (269R) | CGAGCCTGAGCGAACAACAA | |||
| Selected denitrification genes | ||||
| qnarG1-F | AGCCCACATCGTATCAAGCA | 61 | 149 | Geoffroy et al. (2018) |
| qnarG1-R | CCACGCACCGCAGTATATTG | |||
| narK12 (257F) | TTCTGATCTGCCCGAACTCT | 60 | 106 | Mauffrey, Martineau & Villemur (2015) |
| narK12 (362R) | GCGCCTAGCAATGCTTTTAC | |||
| narG2 (597F) | TTACGCTGCAGGATCACGTT | 60 | 127 | Mauffrey, Martineau & Villemur (2015) |
| narG2 (723R) | TGACTCGGGTACATCGGTCT | |||
| qnirK-F | AAGTCGGTAAAGTAGCCGTTGA | 55 | 138 | Geoffroy et al. (2018) |
| qnirK-R | TCTCCATCGTCATTTGAACAAC |