Skip to main content
[Preprint]. 2024 Oct 24:2024.10.03.616531. [Version 2] doi: 10.1101/2024.10.03.616531

Figure 1: RNA survived in dried flies over 72 weeks.

Figure 1:

(A) Average RNA concentration and (B) length of RNA from dried and frozen flies over 72 weeks. N = 3 female D. melanogaster from each storage condition at each timepoint. (C) Number of samples positive of 3 male and 3 female flies at each timepoint for RpL32 mRNA via RT-qPCR using primers that target long (110 nt) and short (56 nt, dried only) regions of RpL32 mRNA. (D) Average difference of RpL32 mRNA, galbut virus and Nora virus RNA levels relative to fresh D. melanogaster collected at the initial timepoint. A two-tailed t-test was used to determine the statistical significance between dried and frozen means at every time point (A < 0.05, B < 0.001). (E) Comparison of mean Ct value at each time point of dried D. melanogaster using primers that target a long (x-axis) or short (y-axis) region of RpL32 mRNA and galbut virus. Error bars represent mean plus and minus the standard deviation. N = 3 male and 3 female at each time point.