Fig. 2: RAPPL can enrich and purify ribosomes from limited biological material.
A. TEM visualization of RAPPL eluates from PureExpress® ribosomes from a 10-fold dilution scheme of 13.3 μM to 1.3 nM. The scale bar represents 500 nm. B. Western blot analysis using αHA antibody on RAPPL eluates of HEK-293 cells in which uL4 was HA-tagged by CRISPR/Cas9 whereby the starting cells were diluted to 1×106, 5×105, 2.5×105, 1×105, 5×104, 2.5×104, 1×104, 5×103, 1×103, and 0.1×103 cells prior to lysis. C. Western blot analysis using αHA antibody on RAPPL eluates of P. falciparum NF54 cells in which PfRACK was C-terminally tagged with mNeonGreen-HA whereby the starting cells were diluted to 5×107, 1×107, 5×106, and 1×106 cells. Molecular markers indicate size of detected proteins.