Fig. 6: Structural determination of RAPPL products can produce high-resolution CryoEM Maps.

C. neoformans cells (~10⁸) in exponential phase were lysed and the clarified lysate used in RAPPL. The ribosomes were eluted in 30 μL of elution buffer. The Eluate was first screened using TEM. Subsequently, grids were prepared using 5 μL of eluate. Movies were captured on FEI Titan Krios G3 300kV Cryo-TEM with Falcon IV Direct Electron Detection camera. Data was processed using cryoSPARC resulting in an ~2.7 Å global resolution.