Figure 3. Adenylate kinase catalytic parameters span three orders of magnitude and correlate weakly with phylogeny and environmental conditions.

(A) Catalytic parameters and T_Growth values for 100/181 ADK orthologs (including those with K_M outside the range of the assay) are displayed as a heatmap across a taxonomic tree (Methods). k_cat and K_M are colored on a log scale. Orthologs with K_M outside the range of the assay are labeled with asterisks (*). See Fig. S7 for a heatmap of all 181 ADK orthologs. The consensus ADK sequence (94) is plotted at the bottom (k_cat = 5.4 s⁻¹, K_M = 804 uM). (B) Measured k_cat values for 175 orthologs span three orders of magnitude. The vertical dashed line represents the k_cat for the ADK consensus sequence (94). (C,D) Phylogenetic signal analysis of (C) k_cat and (D) T_Growth. Moran’s I index of autocorrelation is plotted as a solid black line, with the 95% confidence interval outlined by dashed black lines. The colored bar at the bottom encodes the significance of autocorrelation, with red and blue representing positive and negative significant autocorrelation, respectively, and black representing nonsignificant autocorrelation. (E) Linear regression analysis between optimal growth temperature and k_cat. The regression line is plotted as a dashed blue line (r² = 0.09, p < 0.001).