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[Preprint]. 2024 Oct 24:2024.10.23.619923. [Version 1] doi: 10.1101/2024.10.23.619923

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Extended Data Figure 6. — Representative electron micrograph and 2D class averages are shown at the start of the flowchart. At the end of the flowchart is the gold-standard Fourier shell correlation curve (0.143 cutoff: horizontal dashed line), angular distribution of particles (heat map), and local resolution estimation plotted on the maps (calculated in CryoSPARC). This flowchart additionally has a summary of local refinement strategies employed for improving local map resolution. CTF: contrast transfer function; NUR: non-uniform refinement.