Figure 6.

More broadly expressed neuropeptide genes. EASI-FISH was used to examine the expression of the indicated neuropeptide genes in the central brain. Samples were counterstained with DAPI to visualize the outline of brain tissue. Images are MIPs. We included spab and Nplp1 in our screening although it is unclear whether these are indeed neuropeptides (M. Zandawala, pers. comm.). A higher magnification view of a region of the brain showing NPF expression (indicated by dashed box in J) is shown in K. Scale bar in each panel = 50 μm; note that the images shown are from brains that were expanded by about a factor of two during the EASI-FISH procedure.