Figure 1: Workflow of transformed NSPCs.
a. Experimental workflow used to collect histological samples and dissociated cells from mice injected with transformed NSPCs.
b. FACS sort scheme to collect isolated NSPCs from fetal human brain. Adapted from Liu et al. 2023. Lineage exclusion included antibodies against CD31, CD34, CD45, CD105, and CD235a.
c. Lentiviral constructs used for TP53R175H-mCherry and shNF1-mKOk double knockout
d. FACS sort scheme to collect mCherry and mKOk double positive cells