Figure 3. Long-term oral SEP treatment prevents mammary tumor occurrence of MMTV-neu mice, while inducing systemic immunological reprogramming.

A. Tumor-free survival curves of MMTV-neu mice treated with control DMSO vs. SEP (1 mg/kg, n=20) in drinking water. B. Major cell types (top) and sub cell types (middle) determined by single cell sequencing of PBMCs of DMSO with tumor, DMSO without tumor, SEP with tumor, and SEP without tumor groups. (Bottom) UMAPs featuring the major cell type for each group. Note the enrichment of T cells in PBMCs of SEP without tumor group. C. Volcano plot for differentially expressed genes in PBMCs of MMTV-neu mice which were SEP-treated and did not develop tumors (SEP without tumors) vs. DMSO-treated and developed tumors (DMSO with tumors). D. Chord diagrams of genes elevated (left) or downmodulated (right) in PBMCs of SEP without tumor group compared to those of DMSO with tumor group