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[Preprint]. 2024 Oct 22:2024.10.21.619493. [Version 1] doi: 10.1101/2024.10.21.619493

Figure 1. KPT-330 treatment increases autophagic degradation in Neuro2a cells.

Figure 1.

A. XPO1 silencing efficiency was measure by qPCR (n=3). B. Proteins in control and silenced Neuro2a cells were immunoblotted and levels of XPO1 and LC3 forms (with Bafilomycin A1) were quantified (n=3, ANOVA, *: P<0.05 vs control). C. Neuro2a cells incubated with different concentrations of KPT-330 for 48 hours followed by a treatment with BafilomycinA1 for 6 hours and proteins were resolved by immunoblotting. LC3 forms were quantified (n=3, ANOVA, *: P<0.05, **: P<0.01). D. Expression of autophagy and lysosomal genes (normalized to housekeeping genes, HKG) in cells treated with vehicle or KPT-330 (1uM) were quantified by qPCR (n=3, *: P<0.05, **: P<0.01, ****: P<0.0001, t-test).