Table 2.
Rescue summary of trus4-15/Dftrus developmental timing and lethality.
| background | rescue by | pupariation days AEL | days rescued | lethality | places express the GAL4 |
|---|---|---|---|---|---|
| w 1118 | n/a* | 5 | n/a | viable | n/a |
| trus4-15/Dftrus | n/a* | 12 | n/a | pre-pupal lethal | n/a |
| trus4-15/Dftrus | da>UAS-Trus ** | 5 | 7 | 89% eclosed# w/o defects, fertile | Ubiquitous [70] |
| trus4-15/Dftrus | en, ci>UAS-Trus ** | 6 | 6 | 41% eclosed# female sterile, oogenesis stops at stage5/6 | en + ci en expression is stronger than ci when expressed together |
| trus4-15/Dftrus | en>UAS-Trus ** | 7 | 5 | pre-pupal lethal | posterior half of imaginal discs, some cells in VNC **** |
| trus4-15/Dftrus | ci>UAS-Trus ** | 9 | 3 | 18% eclosed or half-eclosed# w/severe defects in wings, halteres, legs | anterior half of imaginal discs, neuroepithelial cell lineages in optic lobes, low expression in some cells in VNC **** |
| trus4-15/Dftrus | nub>UAS-Trus | 12 | 0 | pre-pupal lethal | wing/haltere disc (pouch), some cells in central brain, low expression in some cells in leg discs **** |
| trus4-15/Dftrus | pdm2>UAS-Trus | 12 | 0 | pre-pupal lethal | wing/haltere disc (pouch + upper posterior corner of hinge), a restricted area of leg discs, lamina cells in optic lobes, some cells in VNC **** |
| trus4-15/Dftrus | repo>UAS-Trus | 12 | 0 | pre-pupal lethal rescued mobility in wandering stage | pan-glial cells in brain [71] |
| trus4-15/Dftrus | elav>UAS-Trus | 12 | 0 | pre-pupal lethal | pan-neuronal cells in brain [71] |
| trus4-15/Dftrus | e22c>UAS-Trus | 12 | 0 | pre-pupal/pupal lethal some reach to pharate and die | epidermal cells[72], limited cells in CNS (our observation) |
| trus4-15/Dftrus | da>UAS-Dilp8RNAi *** | 7 | 5 | pre-pupal lethal | ubiquitous |
| trus4-15/Dftrus | da>UAS-Xrp1RNAi *** | 8 | 4 | pre-pupal lethal | ubiquitous |
Trus protein was expressed in specific tissues with various GAL4 drivers in trus4-15/Dftrus mutant animals. Graphs of pupariation timing of these rescue lines are shown in Fig.1C for *, in Fig.8A for **, and in Fig. 7D for ***.
#
indicates the percentage of 1st instar larvae that ended up ecloing or half-eclosing as adult flies.
da: daughterless-GAL4, en: engrailed-GAL4, ci: cubitous interruptus-GAL4, nub: nubbin-GAL4, pdm2: pdm2-GAL4, repo: repo-GAL4, elav: elav-GAL4, e22c: e22c-GAL4.
****
Expressions are shown in Fig.5C.