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[Preprint]. 2024 Oct 23:2024.10.23.619891. [Version 1] doi: 10.1101/2024.10.23.619891

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Figure 5. — (A) Driving ds-Tango with Or47b-Gal4 in males labels Or47b-OSNs (cyan, shown in A’), their monosynaptic partner LNs and OPNs (green, shown in A’’), and their disynaptic connections (magenta, shown in A’’’).

(B) Driving ds-Tango with Or47b-Gal4 and genetically restricting disynaptic reporter expression to fru-FLP+ neurons in males labels Or47b-OSNs (cyan, shown in B’), their monosynaptic partner LNs and OPNs (green, shown in B’’), and their fru-FLP+ disynaptic connections (magenta, shown in B’’’). Note the presence of ventral projections from the LH to the AVLP in the disynaptic connections characteristic of AV2b1/b2 neurons (arrowheads).

(C) Driving ds-Tango with Ir84a-Gal4 in males labels Ir84a-OSNs (cyan, shown in C’), their monosynaptic partners LNs and OPNs (green, shown in C’’), and their disynaptic connections (magenta, shown in C’’’).

(D) Driving ds-Tango with Ir84a-Gal4 and genetically restricting disynaptic reporter expression to fru-FLP+ neurons in males labels Ir84a-OSNs (cyan, shown in D’), their monosynaptic partners LNs and OPNs (green, shown in D’’), and their fru-FLP+ disynaptic connections (magenta, shown in D’’’). Note the presence of ventral projections from the LH to the AVLP in the disynaptic connections characteristic of AV2b1/b2 neurons (arrowheads).

Maximum intensity Z-stack projection of whole-mount brains are shown. Dashed lines depict the approximate outline of the fly brains. Scale bars, 50 μm.