Table 2. PCR detection of Tricholoma matsutake DNA by the known specific and universal primers from 9 samples in which the fungal DNA was detected by the TmSP-I-2F/TmSP-I-2R primer pair as shown in Table 1.
| Plant name | Samples1 | Detection of T. matsutake using the following specific PCR primers2 | Homology of the sequence obtained using the following universal PCR primer pair with T. matsutake #84 sequence3 | ||||
| TmF/TmR | MY201f/MY101r | pS1 | pS48/pL281 | ITS1/ITS4B | CNL12 (F)/5SA (R) | ||
| Nagano 139 (seedling) | EMRT | ND | ND | ND | − | ND | 411/412 (99.8 %) |
| Ibaraki 1 (seedling) | ERM | − | − | − | − | ND | 410/412 (99.5 %) |
| ERM* | − | − | ND | ND | 635/664 (95.6 %)** | 420/422 (99.5 %) | |
| Shindai-spore+ (seedling) | EMRT | − | − | − | − | ND | ND |
| Nara-blue 1 (air-layered tree) | EMRT | − | − | ND | − | 791/792 (99.9 %) | 455/456 (99.8 %) |
| Nara-blue 2 (air-layered tree) | EMRT | + | + | + | + | 787/789 (99.7 %) | 422/423 (99.8 %) |
| EMRT | + | + | + | + | 746/748 (99.7 %) | 422/423 (99.8 %) | |
| EMRT | − | + | ND | ND | 741/743 (99.7 %) | 423/424 (99.8 %) | |
| Growing wild seedling | EMRT | − | − | − | − | 746/748 (99.7 %) | 413/420 (96.0 %) |
1 Abbreviation is as in Table 1.
2 +: detection of T. matsutake, −: no detection of T. matsutake, ND: not determined due to unknown amplification pattern.
3 All sequences obtained were highly identical to T. matsutake sequences by BLAST search.
* Ectomycorrhizal root tips from adult pines were included in each sample.
** The sequence was also highly matched to the T. matsutake sequence by BLAST search.