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. 2024 Nov 1;5:48. doi: 10.1186/s43556-024-00212-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Peripheral blood derived natural killer cells (NK) were gradually activated by a feeder-free system in vitro. a The percentage of NK cells (CD3⁻CD56⁺) cultured in vitro at day 14, 21 and 27 was detected by using Flow cytometry. b Mean fluorescence intensity (MFI) of surface receptors NKp44 and NKp46 at different time points during NK cell expansion in vitro. c Growth curve of NK cells. d Comparison of killing activity of NK cells against HCT116 cells with different ratio of effector to target at different expansion time. e Heat map of differentially expressed genes. f Differentially expressed activating and inhibitory receptors of NK cells. One-way ANOVA was used for comparisons between multiple groups. All results are presented as the mean ± SD (n = 3)