Fig. 4.

Local administration of rAd.DCN augments NK cell cytotoxic activity and promotes NK cell infiltration in tumor microenvironment. a NK cell counts and b CD107a expression in peripheral blood detected by flow cytometry at 24 h after the first NK infusion (n = 11). c Concentration of Perforin and IFN-γ in serum analyzed by LEGENDplex™ Human CD8/NK Panel Kit at 24 h after NK cell infusion in the first and second treatment cycle (n = 7). d Quantitative PCR assay for the expression of perforin, IFN-γ, and HIF-1α in tumor tissues (n = 3). One-way ANOVA was used for comparisons between multiple groups. e Immunohistochemical staining for the distribution of CD56-positive cells. f Statistical results of positive area ratio for CD56 (n = 9). Comparisons between the two groups were made using an unpaired t-test. Scale bar = 100 µm. Data presented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001