Figure 3. PTPN1/2 inhibition with K884 enhances myogenic differentiation of Duchenne muscular dystrophy (DMD) muscle stem cells (MuSCs).

(A) Control human MuSCs were differentiated with 2, 5, 10, or 20 μM of K884 or vehicle for 4 d (n = 3 biological replicates per group). Cells were fixed and immunolabeled with an anti-MyHC antibody (magenta). Nuclei were counterstained with DAPI (blue). Immunofluorescence images were merged with phase contrast images. Scale bar represents 150 μm. (B) Differentiation efficiency of (A) was determined by quantifying the nuclear fusion index. (C) DMD patient-derived MuSCs were differentiated and treated as described in (A). (D) Nuclear fusion index of (C). Data are represented as normalized to vehicle-treated cells and as mean ± SD, *P < 0.05 (one-way ANOVA with Fisher’s LSD).