Table 1.
Assay validation parameters of the proposed HPLC method for determination of Paracetamol, Dexketoprofen Trometamol and Rivaroxiban.
| Parameter | PAR | DEX | RIV |
|---|---|---|---|
| Linearitya (µg/mL) | 3.00–45.00 | 0.5–50.00 | 0.15-20.00 |
| Slope | 0.1268 | 0.0978 | 0.1181 |
| Intercept | -0.0257 | -0.0014 | 0.0016 |
| Correlation coefficient(r) | 1 | 1 | 1 |
| Accuracya (Mean ± SD) | 101.20 ± 0.912 | 100.45 ± 1.374 | 100.21 ± 0.850 |
| Intra-dayab (RSD %) | 0.318 | 0.583 | 0.193 |
| Inter-dayab (RSD %) | 0.988 | 0.860 | 0.979 |
| Robustness (RSD %)* |
0.410 c 0.325 d |
0.061 c 1.570 d |
0.514 c 0.086 d |
| LOD(µg/mL)e | 0.531 | 0.095 | 0.047 |
| LOQ(µg/mL)f | 1.608 | 0.289 | 0.143 |
aMean of three determinations.
bRelative standard deviations for concentrations (20.00, 25.00 &35.00 µg/mL) for PAR, (13.00, 20.00 & 30.00 µg/mL) for DEX and (4.00, 10.00 & 20.00 µg/mL) for RIV.
cSmall deliberate changes in the flow rate (1.5 ± 0.1 mL/min), the tried flow rates were (1.4, 1.5 and 1.6 mL/min).
dSmall deliberate changes in wavelength of detection (± 2 nm) were made. The tried wavelengths were 252.0, 254.0 and 256.0.
eLOD = 3.3 × SD of intercept/slope.
fLOQ = 10 × SD of intercept/ slope.