Table 2.

The IC₅₀ and k_cat/K_M calculations of AthDHAD and its mutants

		kcat /KM (s−1mM−1)
Enzyme	IC50 (μM)	DHI	DHMV
AthDHAD	0.249 ± 0.000	1.185 ± 0.189	1.622 ± 0.143
I177L	0.050 ± 0.001	0.270 ± 0.015	0.280 ± 0.014
I177F	37.000 ± 8.77	0.023 ± 0.003	0.027 ± 0.007
I177W		N/A
V178L	0.974 ± 0.064	0.153 ± 0.015	0.154 ± 0.004
V178I	0.431 ± 0.016	1.626 ± 0.491	1.077 ± 0.136
V178F	5.991 ± 0.185	0.010 ± 0.001	0.019 ± 0.003
V178W	2.337 ± 0.027	0.009 ± 0.004	0.002 ± 0.000
V496L	0.824 ± 0.004	1.897 ± 0.233	0.983 ± 0.106
V496I	1.140 ± 0.052	0.306 ± 0.060	0.148 ± 0.031
V496F	938 ± 0.012	0.624 ± 0.216	0.249 ± 0.240
V496W		N/A
V497L	0.059 ± 0.000	1.399 ± 0.034	1.070 ± 0.146
V497I	0.073 ± 0.001	0.882 ± 0.095	0.545 ± 0.027
V497F	1.246 ± 0.006	0.051 ± 0.006	0.036 ± 0.008
V497W		N/A
I177L/V496L	0.309 ± 0.074	1.936 ± 0.260	0.760 ± 0.065
I177L/V497L	0.028 ± 0.000	0.107 ± 0.003	0.120 ± 0.004
V178L/V497L	0.396 ± 0.011	0.460 ± 0.018	0.642 ± 0.010

N/A, the biochemical data was not obtainable