Fig. 1. Schematic diagram of chemical strategy to selectively label LD membranes.

a Schematic diagram of the LD chemical labeling strategy. Traditionally, LD labeling strategies using small molecule fluorescent probes mainly focused on labeling neutral lipid nuclei, resulting in spherical-like LD to check the dynamics of content. To achieve a dynamic description of multi-LD membrane dynamics, we propose a approach that involves labeling membrane proteins with a small molecule fluorescent probe, specifically yielding ring-like LD structures. b Schematic diagram of the chemical probe labeling mechanism for LD membrane structure. The three-pronged molecular probe design strategy employs a pro-probe called LDM, which combines lipid enhanced response, microenvironment activation, and electrostatic interaction. This comprehensive approach enables the specific delivery of LDM to the vicinity of LD membranes. Upon activation by the HClO/ClO⁻ microenvironment, LDM-OH becomes exposed and binds to LD membranes through electrostatic interaction, facilitating labeling of LD membrane structure. Image created with Photoshop and Microsoft PowerPoint.