Table 3.

The results of the differential methylation region analysis

Chr	MTinnitus	MNoTinnitus	Change (%)	Gain/loss	Adj p (FDR)	Genes	Gene distance
chr6	2.08	2.58	19.4	Loss	0.0018	HLA-DPB2	Within DMR*
chr1	− 0.43	0.15	373.8	Loss	0.0026	PM20D1	Within DMR
chr2	1.31	2.03	35.4	Loss	0.0040	TMEM18, SNTG2	238,432, 3734
chr3	0.76	1.37	44.0	Loss	0.0065	MUC4	Within DMR*
chr2	− 3.69	− 4.19	11.9	Gain	0.0066	LCLAT1	Within DMR
chr5	− 1.41	− 1.10	28.3	Loss	0.0096	miR886	Within DMR
chr8	− 4.15	− 3.76	10.3	Loss	0.0102	miR596	Within DMR
chr12	− 3.49	− 3.13	11.4	Loss	0.0117	TXNRD1, EID3	Within DMR
chr21	− 2.04	− 2.43	15.9	Gain	0.0127	RUNX1	Within DMR
chr5	− 3.65	− 3.14	16	Loss	0.0139	SDHAP3	Within DMR
chr5	− 3.17	− 3.49	9.3	Gain	0.0150	RUFY1	Within DMR
chr5	− 3.61	− 4.05	10.9	Gain	0.0170	NUDT12	Within DMR
chr15	− 4.12	− 4.49	8.2	Gain	0.0190	TTC23	Within DMR
chr6	1.57	1.88	16.6	Loss	0.0217	HLA-DPB2	Within DMR*
chr15	1.10	1.51	27.0	Loss	0.0235	LASS3 (CERS3)	Within DMR
chr17	− 0.40	− 0.84	52.3	Gain	0.0249	SLC43A2	Within DMR
chr10	1.40	1.91	26.9	Loss	0.0273	C10orf11 (LRMDA)	Within DMR
chr6	− 3.57	− 3.29	8.7	Loss	0.0335	HLA-DQB1	Within DMR*
chr1	3.27	3.83	14.5	Loss	0.0348	NADK	Within DMR
chr4	− 4.78	− 5.09	6.1	Gain	0.0381	C4orf37 (STPG2)	Within DMR
chr1	− 2.73	− 2.23	22.4	Loss	0.0391	SZRD1, MFAP2	6045, 12
chr1	1.84	2.31	20.5	Loss	0.0424	NUP210L, TPM3	Within DMR
chr8	− 5.66	− 5.25	7.8	Loss	0.0425	INTS9	Within DMR
chr12	− 5.04	− 5.60	10.0	Gain	0.0474	EFCAB4B	Within DMR
chr12	− 3.84	− 3.49	9.7	Loss	0.0474	SLC2A14	Within DMR

^*DMR within the region typically not included in the genome-wide association studies. These associations are at a high risk of being false-positive hits (see Supplementary File S1)

M_Tinnitus and M_NoTinnitus present average M-values for CpG probes within DMRs for tinnitus and no tinnitus groups, respectively. Gain (or hypermethylation) was defined as an average M-value higher (more positive) for the tinnitus group than no tinnitus group. Loss (or hypomethylation) was defined as an average M-value lower (less positive) for the tinnitus group than no tinnitus group