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Sequences of primers used for quantitative methylation specific PCR.
| Gene | Primer | Sequence (5′-3′) |
|---|---|---|
| MGMT promoter | M-forward | TTTCGACGTTCGTAGGTTTTCGC |
| M-reverse | GCACTCTTCCGAAAACGAAACG | |
| U-forward | TTTGTGTTTTGATGTTTGTAGGTTTTTGT | |
| U-reverse | AACTCCACACTCTTCCAAAAACAAAACA |
M Methylated; U, Unmethylated.
