qRT-PCR validation of DEGs related to the biosynthesis pathways of theanine (A), catechin (B), caffeine (C), gibberellins (D-E), and indole-3-acetic acid (IAA) (F-G) in tea leaves under different spreading durations. Bar graphs represent qRT-PCR results, while dashed line graphs show the fragments per kilobase of transcript per million mapped reads (FPKM) values from RNA-Seq analysis. Different uppercase letters indicate significant FPKM differences, and lowercase letters indicate significant qRT-PCR differences (one-way ANOVA test; P < 0.05).