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. 2024 Oct 17;146(43):29305–29310. doi: 10.1021/jacs.4c11213

Figure 2.

Figure 2

MaCXE mediated yield increase via removal of the C21 acetoxy group. Proposed reactions and accumulation of intermediates demonstrating MaCXE activity. Accumulation represented by integrated peak areas (based on extracted ion chromatograms (EICs)) for intermediates produced by transient expression in N. benthamiana (Figure S5). Biosynthetic genes for production of epi-neemfruitin B (3) (gray box) were expressed with stepwise addition of the downstream pathway genes (MaL7AT, MaAKR, MaCYP716AD4 and MaLFS), with and without MaCXE (filled and unfilled circles, respectively). Mean values ± SE (n = 6).