Figure 4.

Activities of selected ONs in HeLa pLuc/705 cells. (A) ONs were transfected into HeLa pLuc/705 cells at the indicated concentrations using Lipofectamine 2000, and luciferase activity was measured 48 h later. (B) ONs were applied to HeLa pLuc/705 cells at the indicated concentrations in the absence of a transfection reagent, and luciferase activity was measured 72 h later. In all cases, luminescence was normalized to total protein quantity and untreated cells. Data are means ± standard deviations for three biological replicates (n = 3), where each biological replicate was performed in technical triplicate. Statistics are two-way analysis of variance (ANOVA) with Dunnett’s multiple comparisons test against ON44, α = 0.05: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, and ****P ≤ 0.0001. None of the oligonucleotides in this study displayed any significant toxicity as judged by cell growth (Supporting Information Figure S228).