Table 1. Oligonucleotides Used and Duplex Melting data^a.

^aNucleotides in black have 2′-O-Me ribose sugars and phosphorothioate internucleoside linkages. Nucleotides in red are locked nucleic acid phosphothiotriesters (except ON17–ON24 which have phosphorothioate internucleoside linkages), the red ‘o’ indicates phosphotriester linkage instead of phosphothiotriester. ΔTm = difference in duplex melting temperature of ON1–ON41 against DNA and RNA compared to control ON44 (2′-O-methyl phosphorothioate). Tm of control vs DNA = 48.5 °C, Tm of control vs RNA = 61.3 °C. Melting temperatures were recorded in 10 mM Na-phosphate buffer, pH = 7.0. The melting buffer for complementary DNA contained additional 100 mM NaCl and the melting buffer for complementary RNA contained additional 25 mm NaCl. Tm values used for the ΔTm calculations are an average of three experiments with an error of ±0.25 °C. Comprehensive melting temperature data is in supporting information 4.0, Tables T6–T17. In some cases, the Tm against complementary RNA was too high to determine so additional Tm data was obtained in 10 mM Na-phosphate buffer, pH = 7.0 with no additional NaCl. These Tm values were adjusted using the following tool: http://biotools.nubic.northwestern.edu/OligoCalc.html. These ΔTm values are in red. See Supporting Information4.8 for melting curves without additional NaCl.