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. 2024 Oct 14;20(14):5594–5607. doi: 10.7150/ijbs.96089

Figure 5.

Figure 5

Downregulation of TRIM21 increases OAS3 protein in sepsis. (A) Western blot analysis of TRIM21 protein level in MLE12 cells treated with LPS. (n=6). (B) Western blot analysis of TRIM21 protein level in lung tissue of Sham and CLP mice. (n=3). (C) MLE12 cells were treated with or without LPS and then the interaction of OAS3 and TRIM21 was measured by Co-IP assay. (D) Co-IP assay analysis of the interaction between OAS3 and TRIM21 in lung tissue of Sham and CLP mice. (n=3). (E) Western blot analysis of OAS3 protein in MLE12 cells transfected with the TRIM21 overexpression plasmids followed by LPS treatment. (n=4). (F) Western blot analysis of OAS3 protein in lung tissue of mice intratracheally injected with the empty vector (EV) or TRIM21 overexpression AAV9 followed by CLP operation (n=3). (G) After being transfected with the TRIM21 overexpression plasmid, MLE12 cells were treated with or without MG132 plus LPS treatment, and then the OAS3 protein level was detected by Western blotting (n=4). Data are shown as Mean ± SD. **P<0.01, ***P<0.001.